Vladimir I. Potkin, Sergey K. Petkevich, Alexander S. Lyakhov, Ludmila S. Ivashkevich.
Mononuclear heterocyclic rearrangement of 5-arylisoxazole-3-hydroxamic acids into 3,4-substituted 1,2,5-oxadiazoles
V.I. Potkin, A.V.Kletskov, S.K.Petkevich, S.G.Pashkevich, V.V.Kazbanov, A.A.Denisov, V.A.Kulchitsky
Synthesis of water soluble isoxazol-3-yl(isothiazol-3-yl) carboxamides and ureas containing amino acid residues – potential anticancer agents
Каранкевич Е.Г., Агабалаев А.А., Попова О.П., Куваева З.И.
Экстракция 20-гидроксиэкдизона из травы левзеи сафлороводной
Semini G., Hildmann A., Klein A., Lucka L., Schön M., Schön M. P., Shmanai V., Danker K.
Inositol-C2-PAF down-regulates components of the antigen presentation machinery in a 2D-model of epidermal inflammation
Convenient preparation of fluorogenic hairpin DNA probes (molecular beacons) carrying a pair of FAM fluorophores (located close to 5′-terminus of the probe) or a pair of BHQ1 quenchers on 3′-terminus (with (BHQ1)2 or BHQ1–BHQ1 composition) is reported. These probes were used for the first time in a real-time PCR assay and showed considerable improvements in fluorogenic properties (the total fluorescence increase or signal-to-background ratio) in assay conditions vs. conventional one-FAM-one-BHQ1 molecular beacon probes as well as vs. hydrolyzable one-FAM-one-BHQ1 TaqMan probes. At the same time, such multiple modifications of the probe do not influence its Cq (a fractional PCR cycle used for quantification). The probe MB14 containing a BHQ1–BHQ1 pair showed a PCR fluorescence/background value of 9.6 which is more than two times higher than that of a regular probe MB2 (4.6). This study demonstrates prospects for the design of highly fluorogenic molecular beacon probes suitable for quantitative real-time PCR and for other potential applications (e.g. intracellular RNA detection and SNP/mutation analysis).
Analyst 2014, 139, 2867–2872
|© 2009-2012 Институт физико-органической химии НАН Беларуси||Разработка и поддержка сайта ConstFlash|